֬ Ni BerpharoseFF His tag鰱ǩ״

۸ ¥ 700.00 ¥ 560.00
1-9 ≥10
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ѷ

      ѡ0/0.00Ԫ

      Ƿ ϡ׺Ͳʡ
      BTR206Q-5ml ԼLR 100
      Ʒ 5mlԤװ CAS / Ʒ bersee
      ;Χ ״ȿСѧ Ʒ ֬FF ǷΣջѧƷ

      -֬FF

      Ni-Berpharose FF

      1.      Ʒ

      -֬FF֬΢ΪʣżǰᣨIDA֮Ni2+IDAǽϲõ壬ʹεᣨNTAȣIDA׺ǿߡظʹõŵ㡣

      -֬FFҪڴ鰱ǩHis-Tag鵰ס鰱ǩеͬɽNi2+γȶλ졢ι̺Ϳڹ̶Ni2+ĻϣͨŨȶ鵰׽оϴѡ

      2.

      1mlԤװ5mlԤװ10mlԤװ20ml100ml500ml

      3.Ʒ

      ָ

      6%֬΢

      -N(CH2COOH)2

      ܶ

      30~40μmol/ml

      40mg Hisǩ/ml

      45~165μm

      Ƽ/

      50~100/700 cm/h

      ͷѹ

      0.3MPa

      4~40

      pHȶΧ:/

      5~12/3~14

      滺Һ

      20%Ҵ

      4-8

      4.

      ƽ

      -֬FF2~5ijʼҺƽ⡣

      Ϊ100 cm/h

      1ƷһܽpH6~8ijʼҺСҺpHֵ

      2ҺвܺEDTAΣͬʱñϻҴDTTȻԭ

      3ûҺ10~100 mMƻҺ20~200 mM Tris-HClҺȡ

      4ҺһҪ0.15~0.5 MNaClӽá

      5ʹ-֬FFʱƼʹ50 mM PBS50 mM NaH2PO40.5 M NaCl,pH 7.4ΪʼҺ

      ϴ

      1ŨȽϴ-֬FFõϴѷ

      2ΪԣӦҺƺҪHClpHֵ

      3ʹ-֬FFʱ粻ȷϴҪŨȣƼڳʼҺзֱ10 mM20 mM50 mM100 mM200 mM500 mMŨȴӵ

      ߷ֱϴѺռ鵰ף֮ͨSDS-PAGEӾȷϴѽ

      4ĿԽݶϴȷϼѵϴ

       

      ϴѷ

      1pHϴѣpH6~4ᱻϴҲpH 3~4ҺǴơλϵ

      2ϴѣӻһͬ׺ľʵŨȣ0~0.5M0~50 mM鰱ᡢ0~2M NH4ClݶϴʼҺĺ㶨pHֵ½С

      3ϼϴѣEDTAEGTAϼͬӲµױϴ˷ܷ벻ͬĵףӰ쵰׵ںϵ޷

      עpHϴѺϼϴѻʹý䣬´ʹǰҪͽӡõķΪϴѡ

      ϴѷڻҺм150 ~500mMNaClӽá

       

      5.ϴ

      1ʹúҪϵĽʱ뽫5~10ˮϴӣ5~10100 mM EDTAϴӣ2~30.5 M NaClϴEDTA

      2ʹõһҪϴϴ0.1~1.0 M NaOHϴӣ50 cm/h1~2 hȥǿʣͬʱҲȥԴ

      3ϴҪϽӡϷ2~5ˮƽӣ0.1~0.3 MҺ5~10Ͻӣ֮5~10ˮϴȥδϵĽӡ

      λϴ

      1ȥӽĵף2~32 M NaClҺϴ

      2ȥǿˮԵ׺֬ʵȣ470%Ҵ30%ϴ

      3ȥ׳ˮԵף衣

       

      6.ע

      1)ʹʱ֤ӺͻҺ¶һ£ڲݣӰ촿Ч

      2)ƷΪ20%Ҵ4~8

      3) 2-25,ѹ,ܹ

      4ʵʹ

       

       

      (Ԥװ)Ŀ¼

      ׺Ͳ(Ԥװ) 

      ﵰA֬FF (Portein A Berpharose FF)

      ﵰG֬FF (Portein G Berpharose FF)

      ͼA֬FF (rat-Portein A Berpharose FF)

      ﵰAG֬FF (Portein AG Berpharose FF)

      -֬FF (Ni- Berpharose FF)

      ù׺֬FF (Streptavidin Berpharose FF)

      Strep-Tactin֬FF (Strep-Tactin Berpharose FF) (StrepIIǩ״)

      -֬FF (Heparin- Berpharose FF)

      ȵøƼ֬FF (STI- Berpharose FF)

      ڶ֬(Endotoxin Removal Reagent Berpharose)

      GST-֬FF (GST Berpharose FF)

      -֬FF (Co- Berpharose FF)

      Ѫ׵֬FF (HSA- Berpharose FF)

      ԴѪƤ׺֬FF (rh-VEGF Berpharose FF)

      ˼Գάϸ׺֬FF (rh-bFGF Berpharose FF)

       

      ؼ׺

      ùأBG塢M1B1-׺(AFT-IAC)

      ùA-׺(OTA-IAC)

      ׳ùϩͪ-׺(ZEA-IAC )

      ѩϩŻ¶أ-׺(DON-IAC)

      T-2-׺(T-2 toxin-IAC)

      -׺(FB1FB2FB3-IAC)

       

      NHS֬FF (NHS Activated Berpharose FF)

      ֬FF ( Epoxy Activated Berpharose FF)

       

      ӽ(Ԥװ)

      DEAE-֬FFDEAE Berpharose FF

      Q-֬FFQ Berpharose FF

      CM-֬FFCM Berpharose FF

      SP-֬FFSP Berpharose FF

      DEAEQCMSPԤװװ4֧

       

      ˮ(Ԥװ)

      -֬FFPhenyl Berpharose FF

      -֬FFOctyl Berpharose FF

      -֬FFButyl Berpharose FF

       Ԥװװ3֧


      ˲(Ԥװ

      ͺ뷶Χ
      G10<700
      G15100-1500
      G25 ()1000-5000
      G501000-30000
      G753000-80000
      G1004000-120000
      G1505000-300000
      G2005000-600000

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